Until recently, PCR was the only method for direct isolation of desirable chromosomal segments from complex genomes. Although it is widely used for the analysis of genomes, PCR has one major ...
One of the most indispensable techniques in all areas of the life sciences is the use of expression vectors. Cloning a gene between two restriction sites, and the expression of a protein, are almost ...
SAN DIEGO--(BUSINESS WIRE)--SGI-DNA announces the launch of the Multi-Tile Assembly Cloning (MTAC) application for its BioXp™ 3200 System, providing scientists with the ability to synthesize two genes ...
To reduce the time dedicated to clone screening in the development of cell lines for stable virus production, a protocol merging the procedures of cell cloning and titer screening was implemented: the ...
Developing cell lines for use as accurate models for disease has always been a challenging task. In order to replicate phenotypes or enable downstream experimentation, genome engineering of cell lines ...
Molecular cloning refers to the replication and recombination of DNA molecules. The first cloning experiments were carried out in the 1970s, when restriction endonucleases were discovered. Restriction ...
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