To obtain high-quality, purified nucleic acids, scientists can use several different methods, including phenol-chloroform extraction, cesium chloride/ethidium bromide (CsCl/EtBr) gradient ...

Real-time PCR (qPCR) analysis has become the method of choice for quantitating DNA in a wide variety of applications. The availability of commercial kits has made the technique easy to perform, ...

Morgan Hiebert (left) studies drug-resistant tuberculosis within the National Reference Centre for Mycobacteriology (Winnipeg, Manitoba) led by Hafid Soualhine, situated in the National Microbiology ...

Starting from whole plant tissues and dissociating cells (protoplasts) or nuclei from the tissue, RNA extraction, cDNA synthesis by reverse transcription, library preparation followed by sequencing, ...

Transcriptomics represents a critical discipline in cancer research, enabling comprehensive mapping of gene expression profiles and the identification of fusion genes implicated in tumor development.

RNA sequencing enables sensitive fusion detection and direct assessment of transcript-level disruption caused by splicing mutations. In the study, "Clinical utility of targeted RNA sequencing in ...

In this issue of BioTechniques, we present the development of a novel antibody for the detection of fMet-bearing proteins across different species, a method for characterizing the microbiome ...